chemiluminescence (method of)

Chemiluminescence is the light emitted during a chemical reaction. Its measurement makes possible the overall detection of active oxygen species produced without identifying them. This method is used without an enhancer molecule for the study of certain chemical reactions, especially those involving peroxynitrite or singlet oxygen. In the presence of the enhancer lucigenin, it is used to evaluate the oxidant cellular activity, particularly that of the polymorphonuclear leukocytes.