The Cuban strain
A collaboration between Cuban researchers, a virologist from KU Leuven and Professor Michel Moutschen from GIGA at the University of Liège has led to the identification of a particularly aggressive strain of HIV in Cuba. In a study published in EBioMedicine, the scientists reveal how the CRF19 strain causes the rapid progression of the infection to AIDS.
From intuition to collaboration
We often talk about the Human Immunodeficiency Virus as if there was only one type. In reality, several well-identified types, groups and subtypes of this virus coexist in the world. Owing to HIV’s great ability to mutate, the number of different strains is increasing. Sometimes, a person can be infected by two separate strains of HIV which then recombine, meaning their genome is “mixed”. Such an event can therefore give rise to a new strain of HIV. “These new strains may have surprises in store for us by developing new properties. That’s why it’s important to monitor the situation”, explains Professor Michel Moutschen, Director of GIGA’s Immunology and Infectious Diseases Research Unit at ULg, and Head of Department at CHU Liège’s Centre de référence SIDA. Within the framework of a collaboration with KU Leuven and Cuban researchers, Michel Moutschen participated in the characterisation of a particularly virulent recombinant HIV strain present in Cuba. “Since the beginning of the 2000s, Cuban researchers had the intuition that local patients infected by HIV were progressing very rapidly to AIDS”, Michel Moutschen points out. "Thanks to funding from the Walloon Region, Anne-Mieke Vandamme, an eminent AIDS virologist from KU Leuven, and I were able to welcome a Cuban researcher, Vivian Kouri, and train her in a series of lab techniques used for this study", he explains. This team work helped them to discover the CRF19 strain present in Cuba, which is indeed associated with a rapid progression to the disease in certain patients. The results of this study were published in the journal EBioMedicine (1) and caused quite a stir at the annual CROI (Conference on Retroviruses and Opportunistic Infections) last February.
HIV, a two-cycle pathogen
In the majority of cases, HIV strains have a tropism that remains limited to the T lymphocytes of the mucous membranes during the first few years of the infection. “When contamination occurs, and during the initial period of infection, the virus is configured in such a way that it can bind to the CD4 T lymphocytes of the mucous membranes and, in particular, the intestinal mucous membranes”, Michel Moutschen elaborates. These lymphocytes present a certain type of receptor on their surface which isn’t the same as those found on other T lymphocytes in the body, for instance in the spleen, in ganglions or in the blood. “Hence, for a certain period of time, the damage linked to the presence of the virus is limited to the T lymphocytes of the mucous membranes and the rest of the immune system is protected”, the specialist continues. “This is what explains the asymptomatic phase of the infection, which can last several years. The virus is present, replicates but the person is in good health and presents only very low concentrations of infected T lymphocytes in the blood”, Michel Moutschen adds. In general the first signs of illness due to HIV only appear after five or six years. “Then the virus mutates and acquires the ability to bind to the T lymphocyte receptors present in the whole body. At this point, the patient’s immunity drops sharply with the onset of AIDS. That’s the usual scenario”, the researcher specifies.
(1) Vivian Kouri, Ricardo Khouri, Yoan Alemán, Yeissel Abrahantes, Jurgen Vercauteren, Andrea-Clemencia Pineda-Peña, Kristof Theys, Sarah Megens, Michel Moutschen, Nico Pfeifer, Johan Van Weyenbergh, Ana B. Pérez, Jorge Pérez, Lissette Pérez, Kristel Van Laethem, Anne-Mieke Vandamme. CRF19_cpx is an Evolutionary fit HIV-1 Variant Strongly Associated With Rapid Progression to AIDS in Cuba. EBioMedicine. http://dx.doi.org/10.1016/j.ebiom.2015.01.015